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Product Description
Human Ribonuclease P protein subunit p29 (POP4) ELISA Kit | KTE62350 | Abbkine
Application: This Human Ribonuclease P protein subunit p29 (POP4) ELISA Kit employs a two-site sandwich ELISA to quantitate POP4 in samples. An antibody specific for POP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Ribonuclease P protein subunit p29 microplate
• Human Ribonuclease P protein subunit p29 standard
• Human Ribonuclease P protein subunit p29 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Ribonuclease P protein subunit p29 (POP4) ELISA Kit has high sensitivity and excellent specificity for detection of Human POP4. No significant cross-reactivity or interference between Human POP4 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: POP4 encodes one of the protein subunits of the small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is localized to the nucleus and associates directly with the RNA component of these complexes. This protein is involved in processing of precursor RNAs. Alternative splicing results in multiple transcript variants.The deduced 220-amino acid basic protein is rich in arginine and lysine, has nuclear localization sequences, and has a predicted molecular mass of approximately 25 kD. RPP29 is 23% identical to the yeast Pop4 protein. Fluorescent microscopy demonstrated nuclear and nucleolar expression of RPP29. Immunoblot analysis showed that antibodies to RPP14 or RPP29 precipitate native RNase P.
Alternative Names: POP4; RPP29; processing of precursor 4; ribonuclease P protein subunit p29
Search name: POP4; RPP29; processing of precursor 4; ribonuclease P protein subunit p29
Tag: POP4
