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Product Description
Human Secretory leukocyte protease inhibitor (SLPI) ELISA Kit | KTE62605 | Abbkine
Application: This Human Secretory leukocyte protease inhibitor (SLPI) ELISA Kit employs a two-site sandwich ELISA to quantitate SLPI in samples. An antibody specific for SLPI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLPI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLPI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLPI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Secretory leukocyte protease inhibitor microplate
• Human Secretory leukocyte protease inhibitor standard
• Human Secretory leukocyte protease inhibitor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Secretory leukocyte protease inhibitor (SLPI) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLPI. No significant cross-reactivity or interference between Human SLPI and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Antileukoproteinase is secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also thought to have broad-spectrum anti-biotic activity.Secretory leukocyte protease inhibitor (SLPI) is a highly cationic single-chain protein with eight intramolecular disulfide bonds. It is found in large quantities in bronchial, cervical, and nasal mucosa, saliva, and seminal fluids. SLPI inhibits human leukocyte elastase, human cathepsin G, human trypsin, neutrophil elastase, and mast cell chymase.
Alternative Names: SLPI; ALK1; ALP; BLPI; HUSI; HUSI-I; MPI; WAP4; WFDC4; WAP four-disulfide core domain 4; antileukoproteinase; mucus proteinase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase) ; seminal
Search name: SLPI; ALK1; ALP; BLPI; HUSI; HUSI-I; MPI; WAP4; WFDC4; WAP four-disulfide core domain 4; antileukoproteinase; mucus proteinase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase) ; seminal
Tag: SLPI
