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Product Description
Human Stromelysin-3 (ST3) ELISA Kit | KTE61600 | Abbkine
Application: This Human Stromelysin-3 (ST3) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP11 in samples. An antibody specific for MMP11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Stromelysin-3 microplate
• Human Stromelysin-3 standard
• Human Stromelysin-3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Stromelysin-3 (ST3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MMP11. No significant cross-reactivity or interference between Human MMP11 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by ST3 is activated intracellularly by furin within the constitutive secretory pathway. Also in contrast to other MMP's, this enzyme cleaves alpha 1-proteinase inhibitor but weakly degrades structural proteins of the extracellular matrix.
Alternative Names: MMP11; SL-3; ST3; STMY3; matrix metalloproteinase 11; matrix metalloproteinase 11 (stromelysin 3) ; stromelysin 3; stromelysinI
Search name: MMP11; SL-3; ST3; STMY3; matrix metalloproteinase 11; matrix metalloproteinase 11 (stromelysin 3) ; stromelysin 3; stromelysinI
Tag: MMP11
