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Product Description
Mouse 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2 (PLCG2) ELISA Kit | KTE70731 | Abbkine
Application: This Mouse 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2 (PLCG2) ELISA Kit employs a two-site sandwich ELISA to quantitate PLCG2 in samples. An antibody specific for PLCG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLCG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLCG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLCG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2 microplate
• Mouse 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2 standard
• Mouse 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2 (PLCG2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLCG2. No significant cross-reactivity or interference between Mouse PLCG2 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Overexpressed wildtype rat Plcg1 or a lipase-inactive mutant Plcg1 each augmented ACE in rat PC12 cells, while a deletion mutant lacking the region containing the SH3 domain of Plcg1 was ineffective. RNA interference to deplete either Plcg1 or Plcg2 in PC12 and rat aortic smooth muscle A7r5 cells inhibited ACE. In chicken DT40 B lymphocytes expressing only Plcg2, overexpressed human muscarinic M5 receptors (M5R) activated ACE. Using DT40 PLC2 knockout cells, M5R stimulation of endoplasmic reticulum Ca (2+) store release was unaffected, but ACE was abolished. Normal ACE was restored by transient expression of rat Plcg2 or a lipase-inactive Plcg2 mutant. The results indicated a lipase-independent role of PLCG in the physiologic agonist-induced activation of Ca (2+) entry.
Alternative Names: PLCG2; Phospholipase C gamma 2; phospholipase C; gamma 2; phospholipase C; gamma 2 (phosphatidylyinositol-specific)
Search name: PLCG2; Phospholipase C gamma 2; phospholipase C; gamma 2; phospholipase C; gamma 2 (phosphatidylyinositol-specific)
Tag: PLCG2
