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Product Description
Mouse Calcium-dependent phospholipase A2 (PLA2G5) ELISA Kit | KTE70739 | Abbkine
Application: This Mouse Calcium-dependent phospholipase A2 (PLA2G5) ELISA Kit employs a two-site sandwich ELISA to quantitate PLA2G5 in samples. An antibody specific for PLA2G5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Calcium-dependent phospholipase A2 microplate
• Mouse Calcium-dependent phospholipase A2 standard
• Mouse Calcium-dependent phospholipase A2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Calcium-dependent phospholipase A2 (PLA2G5) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLA2G5. No significant cross-reactivity or interference between Mouse PLA2G5 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: PLA2G5 is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L- alpha-1-stearoyl-2-arachidonyl phosphatidylinositol.
Alternative Names: PLA2G5; DKFZp686C2294; GV-PLA2; MGC46205; PLA2-10; hVPLA (2) ; Ca2+-dependent phospholipase A2; phosphatidylcholine 2-acylhydrolase
Search name: PLA2G5; DKFZp686C2294; GV-PLA2; MGC46205; PLA2-10; hVPLA (2) ; Ca2+-dependent phospholipase A2; phosphatidylcholine 2-acylhydrolase
Tag: PLA2G5
