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Product Description
Mouse Cyclin-D1 (CCND1) ELISA Kit | KTE71404 | Abbkine
Application: This Mouse Cyclin-D1 (CCND1) ELISA Kit employs a two-site sandwich ELISA to quantitate CCND1 in samples. An antibody specific for CCND1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCND1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCND1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCND1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Cyclin-D1 microplate
• Mouse Cyclin-D1 standard
• Mouse Cyclin-D1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Cyclin-D1 (CCND1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CCND1. No significant cross-reactivity or interference between Mouse CCND1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Cyclin D1 belongs to the family of cyclin proteins which function as the regulatory subunits of cyclin/cyclin dependent kinase (Cdk) holoenzymes that regulate entry into and progression through the cell cycle. Cyclin D1 expression is induced upon stimulation by growth factors (e.g.EGF, IGF-I/II), amino acids, hormones, and oncogenes such as Ras, Src, ErbB2, and SV40 T antigen. Cdk4 and Cdk6 can partner with Cyclin D1 in early to mid-G1 phase to phosphorylate and inactivate the cell-cycle inhibitory function of the retinoblastoma protein (pRB) in cooperation with Cyclin E/Cdk2. Cyclin D1 is also known to modulate local chromatin structure and transcription of genes involved in proliferation and differentiation through CDK-independent association with histone acetylases (e.g. CBP, P/CAF) and deacetylases.
Alternative Names: CCND1; BCL1; D11S287E; PRAD1; U21B31; B-cell CLL/lymphoma 1; G1/S-Specific Cyclin-D1; Parathyroid Adenomatosis 1;
Search name: CCND1; BCL1; D11S287E; PRAD1; U21B31; B-cell CLL/lymphoma 1; G1/S-Specific Cyclin-D1; Parathyroid Adenomatosis 1;
Tag: CCND1
