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Product Description
Mouse DNA (cytosine-5) -methyltransferase 3B (DNMT3B) ELISA Kit | KTE71227 | Abbkine
Application: This Mouse DNA (cytosine-5) -methyltransferase 3B (DNMT3B) ELISA Kit employs a two-site sandwich ELISA to quantitate DNMT3B in samples. An antibody specific for DNMT3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNMT3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNMT3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNMT3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse DNA microplate
• Mouse DNA standard
• Mouse DNA detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse DNA (cytosine-5) -methyltransferase 3B (DNMT3B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DNMT3B. No significant cross-reactivity or interference between Mouse DNMT3B and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: DNMT3B, is a protein associated with immunodeficiency, centromere instability and facial anomalies syndrome. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Alternative Names: DNMT3B; ICF; M.HsaIIIB; DNA MTase HsaIIIB; DNA cytosine-5 methyltransferase 3 beta; DNA methyltransferase HsaIIIB
Search name: DNMT3B; ICF; M.HsaIIIB; DNA MTase HsaIIIB; DNA cytosine-5 methyltransferase 3 beta; DNA methyltransferase HsaIIIB
Tag: DNMT3B
