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Product Description
Mouse DNA-directed RNA polymerase III subunit RPC6 (POLR3F) ELISA Kit | KTE71445 | Abbkine
Application: This Mouse DNA-directed RNA polymerase III subunit RPC6 (POLR3F) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR3F in samples. An antibody specific for POLR3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLR3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR3F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR3F bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse DNA-directed RNA polymerase III subunit RPC6 microplate
• Mouse DNA-directed RNA polymerase III subunit RPC6 standard
• Mouse DNA-directed RNA polymerase III subunit RPC6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse DNA-directed RNA polymerase III subunit RPC6 (POLR3F) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POLR3F. No significant cross-reactivity or interference between Mouse POLR3F and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB) . Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. hRPC39 interacts physically with both hTBP and hTFIIIB90, two subunits of human RNA Pol III transcription initiation factor IIIB. These data strongly suggest that the hRPC32-hRPC39-hRPC62 subcomplex directs RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and hRPC39.
Alternative Names: POLR3F; MGC13517; RPC39; RPC6; DNA-directed RNA polymeraseI 39 kDa polypeptide F; DNA-directed RNA polymerasesI 39 kDa polypeptide; RNA polymeraseI C39 subunit
Search name: POLR3F; MGC13517; RPC39; RPC6; DNA-directed RNA polymeraseI 39 kDa polypeptide F; DNA-directed RNA polymerasesI 39 kDa polypeptide; RNA polymeraseI C39 subunit
Tag: POLR3F
