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Product Description
Mouse E-Selectin (SELE) ELISA Kit | KTE70465 | Abbkine
Application: This Mouse E-Selectin (SELE) ELISA Kit employs a two-site sandwich ELISA to quantitate SELE in samples. An antibody specific for SELE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySELE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse E-Selectin microplate
• Mouse E-Selectin standard
• Mouse E-Selectin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse E-Selectin (SELE) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SELE. No significant cross-reactivity or interference between Mouse SELE and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: CD62E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P) . Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations.
Alternative Names: SELE; RP1-117P20.2; CD62E; ELAM; ELAM1; ESEL; LECAM2; endothelial adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2
Search name: SELE; RP1-117P20.2; CD62E; ELAM; ELAM1; ESEL; LECAM2; endothelial adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2
Tag: SELE
