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Product Description
Mouse Gamma-Aminobutyric Acid IgG autoantibody (GABA-Ab-IgG) ELISA Kit | KTE71517 | Abbkine
Application: This Mouse Gamma-Aminobutyric Acid IgG autoantibody (GABA-Ab-IgG) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GABA-Ab-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated GABA-Ab-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled GABA-Ab-IgG and unlabeled GABA-Ab-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GABA-Ab-IgG in the sample. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Gamma-Aminobutyric Acid IgG autoantibody microplate
• Mouse Gamma-Aminobutyric Acid IgG autoantibody standard
• Mouse Gamma-Aminobutyric Acid IgG autoantibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Gamma-Aminobutyric Acid IgG autoantibody (GABA-Ab-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse GABA-Ab-IgG. No significant cross-reactivity or interference between Mouse GABA-Ab-IgG and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: γ-Aminobutyric acid (GABA) is the chief inhibitory neurotransmitter in the mammalian central nervous system. It plays an important role in regulating neuronal excitability throughout the nervous system. In humans, GABA is also directly responsible for the regulation of muscle tone.In insect species GABA acts only on excitatory nerve receptors. Although chemically it is an amino acid, GABA is rarely referred to as such in the scientific or medical communities, because the term "amino acid, " used without a qualifier, refers to the alpha amino acids, which GABA is not, nor is it incorporated into proteins. In spastic diplegia in humans, GABA absorption by some nerves becomes impaired, which leads to hypertonia of the muscles signaled by those nerves.Gamma-aminobutyric acid was first synthesized in 1883, and was first known only as a plant and microbe metabolic product.
Alternative Names: GABA-Ab-IgG
Search name: GABA-Ab-IgG
Tag: GABA-IgG
