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Product Description
Mouse Multidrug resistance protein 3 (ABCB4) ELISA Kit | KTE70529 | Abbkine
Application: This Mouse Multidrug resistance protein 3 (ABCB4) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCB4 in samples. An antibody specific for ABCB4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCB4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCB4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Multidrug resistance protein 3 microplate
• Mouse Multidrug resistance protein 3 standard
• Mouse Multidrug resistance protein 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Multidrug resistance protein 3 (ABCB4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ABCB4. No significant cross-reactivity or interference between Mouse ABCB4 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Multidrug resistance protein 3 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White) . This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.
Alternative Names: ABCB4; ABC21; GBD1; MDR2; MDR2/3; MDR3; PFIC-3; PGY3; ATP-binding cassette; subfamily B; member 4; OTTHUMP00000210458; P glycoprotein 3/multiple drug resistance 3; P-glycoprotein-3/multiple drug resistance-3;
Search name: ABCB4; ABC21; GBD1; MDR2; MDR2/3; MDR3; PFIC-3; PGY3; ATP-binding cassette; subfamily B; member 4; OTTHUMP00000210458; P glycoprotein 3/multiple drug resistance 3; P-glycoprotein-3/multiple drug resistance-3;
Tag: ABCB4
