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Product Description
Mouse Myosin IG (MYO1G) ELISA Kit | KTE70870 | Abbkine
Application: This Mouse Myosin IG (MYO1G) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1G in samples. An antibody specific for MYO1G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO1G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO1G is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO1G bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Myosin IG microplate
• Mouse Myosin IG standard
• Mouse Myosin IG detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Myosin IG (MYO1G) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYO1G. No significant cross-reactivity or interference between Mouse MYO1G and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: MYO1G is a plasma membrane-associated class I myosin (see MIM 601478) that is abundant in T and B lymphocytes and mast cells. MYO1G (Myosin IG) is a Protein Coding gene. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Delta508-CFTR traffic / ER-to-Golgi in CF. GO annotations related to this gene include actin binding and motor activity. An important paralog of this gene is MYO1D.
Alternative Names: MYO1G; HA2; MGC142104; MHAG; minor histocompatibility antigen HA-2
Search name: MYO1G; HA2; MGC142104; MHAG; minor histocompatibility antigen HA-2
Tag: MYO1G
