Euro
USD
British Pound
NULL
Product Description
Mouse Myosin-XVIIIa (MYO18A) ELISA Kit | KTE70877 | Abbkine
Application: This Mouse Myosin-XVIIIa (MYO18A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO18A in samples. An antibody specific for MYO18A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO18A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO18A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO18A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Myosin-XVIIIa microplate
• Mouse Myosin-XVIIIa standard
• Mouse Myosin-XVIIIa detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Myosin-XVIIIa (MYO18A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYO18A. No significant cross-reactivity or interference between Mouse MYO18A and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The myosin XVIIIA encoded by Myo18a can bind GOLPH3, linking the Golgi to the cytoskeleton and influencing Golgi membrane trafficking. The encoded protein is also part of a complex that assembles lamellar actomyosin bundles and may be required for cell migration.
Alternative Names: MYO18A; DKFZp686L0243; KIAA0216; MYSPDZ; SPR210; SP-A receptor subunit SP-R210 alphaS; myosin 18A; myosin containing PDZ domain
Search name: MYO18A; DKFZp686L0243; KIAA0216; MYSPDZ; SPR210; SP-A receptor subunit SP-R210 alphaS; myosin 18A; myosin containing PDZ domain
Tag: MYO18A
