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Product Description
Mouse Protein disulfide-isomerase A4 (PDIA4) ELISA Kit | KTE70777 | Abbkine
Application: This Mouse Protein disulfide-isomerase A4 (PDIA4) ELISA Kit employs a two-site sandwich ELISA to quantitate PDIA4 in samples. An antibody specific for PDIA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDIA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Protein disulfide-isomerase A4 microplate
• Mouse Protein disulfide-isomerase A4 standard
• Mouse Protein disulfide-isomerase A4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Protein disulfide-isomerase A4 (PDIA4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PDIA4. No significant cross-reactivity or interference between Mouse PDIA4 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Protein Disulfide Isomerase A4is an enzyme in the endoplasmic reticulum in eukaryotes or periplasmic space of prokaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. This allows proteins to quickly find the correct arrangement of disulfide bonds in their fully-folded state, and therefore the enzyme acts to catalyze protein folding. The reduced (dithiol) form of PDI is able to catalyse a reduction of mispaired thiol residues of a particular substrate, acting as an isomerase. Therefore, PDI is capable of catalyzing the posttranslational modification disulfide exchange. Such exchange reactions can occur intramolecularly, leading to the rearrangement of disulfide bonds in a single protein.
Alternative Names: PDIA4; ERP70; ERP72; protein disulfide isomerase A4; protein disulfide isomerase related protein (calcium-binding protein; intestinal-related) ; protein disulfide isomerase-associated 4
Search name: PDIA4; ERP70; ERP72; protein disulfide isomerase A4; protein disulfide isomerase related protein (calcium-binding protein; intestinal-related) ; protein disulfide isomerase-associated 4
Tag: PDIA4
