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Product Description
Mouse Pyruvate dehydrogenase protein X component, mitochondrial (PDHX) ELISA Kit | KTE71455 | Abbkine
Application: This Mouse Pyruvate dehydrogenase protein X component, mitochondrial (PDHX) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHX in samples. An antibody specific for PDHX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDHX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Pyruvate dehydrogenase protein X component, mitochondrial microplate
• Mouse Pyruvate dehydrogenase protein X component, mitochondrial standard
• Mouse Pyruvate dehydrogenase protein X component, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Pyruvate dehydrogenase protein X component, mitochondrial (PDHX) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PDHX. No significant cross-reactivity or interference between Mouse PDHX and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 component of the PDH complex. The open reading frame encodes an 501-amino acid protein sequence that is 20% identical with that encoded by the yeast PDX1 gene and 40% identical with that encoded by the lipoacyltransferase component E2 of the PDH (E2), and includes a lipoyl-bearing domain. Sequence comparisons showed that the PDHX protein contains an E3-binding domain.
Alternative Names: PDHX; DLDBP; E3BP; OPDX; PDX1; proX; dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; pyruvate dehydrogenase complex; E3-binding protein subunit; pyruvate dehydrogenase comp
Search name: PDHX; DLDBP; E3BP; OPDX; PDX1; proX; dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; pyruvate dehydrogenase complex; E3-binding protein subunit; pyruvate dehydrogenase comp
Tag: PDHX
