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Product Description
Mouse Serine/threonine-protein kinase PLK1 (PLK1) ELISA Kit | KTE70729 | Abbkine
Application: This Mouse Serine/threonine-protein kinase PLK1 (PLK1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLK1 in samples. An antibody specific for PLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Serine/threonine-protein kinase PLK1 microplate
• Mouse Serine/threonine-protein kinase PLK1 standard
• Mouse Serine/threonine-protein kinase PLK1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Serine/threonine-protein kinase PLK1 (PLK1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLK1. No significant cross-reactivity or interference between Mouse PLK1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Plk1 consists of 603 amino acids and is 66kDa. In addition to the N-terminus kinase domain, there are two conserved polo-box regions of 30 amino acids at the C-terminus. Plk1 is an early trigger for G2/M transition. Plk1 supports the functional maturation of the centrosome in late G2/early prophase and establishment of the bipolar spindle. Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Plk phosphorylates and activates components of the APC. The APC, which is activated by Fizzy-Cdc20 family proteins, is a cell cycle ubiquitin-protein ligase (E3) that degrades mitotic cyclins, chromosomal proteins that maintain cohesion of sister chromatids, and anaphase inhibitors.
Alternative Names: PLK1; PLK; STPK13; cell cycle regulated protein kinase; polo (Drosophia) -like kinase; polo like kinase; polo-like kinase 1
Search name: PLK1; PLK; STPK13; cell cycle regulated protein kinase; polo (Drosophia) -like kinase; polo like kinase; polo-like kinase 1
Tag: PLK1
