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Product Description
Mouse Transmembrane 4 L6 family member 20 (TM4SF20) ELISA Kit | KTE70202 | Abbkine
Application: This Mouse Transmembrane 4 L6 family member 20 (TM4SF20) ELISA Kit employs a two-site sandwich ELISA to quantitate TM4SF20 in samples. An antibody specific for TM4SF20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM4SF20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM4SF20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM4SF20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Transmembrane 4 L6 family member 20 microplate
• Mouse Transmembrane 4 L6 family member 20 standard
• Mouse Transmembrane 4 L6 family member 20 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Transmembrane 4 L6 family member 20 (TM4SF20) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TM4SF20. No significant cross-reactivity or interference between Mouse TM4SF20 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: TM4SF20, Belongs to the L6 tetraspanin family, Members of the L6 family of membrane proteins, a branch of the tetraspanin superfamily, are overexpressed in tumor cells from many types of cancers. However, direct evidence of their oncogenic activity has not been previously shown. this gene is expressed at low level, only 16.6% of the average gene in this release.The gene contains 4 different gt-ag introns. Transcription produces one mRNA. There are 3 validated alternative polyadenylation sites. The spliced mRNA putatively encodes a good protein, containing L6 membrane domain, some transmembrane domains, apparently vertebrate specific Proteins are expected to localize in various compartments.
Alternative Names: TM4SF20; FLJ22800; PRO994; TCCE518;
Search name: TM4SF20; FLJ22800; PRO994; TCCE518;
Tag: TM4SF20
