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Product Description
Rat Alpha-2-macroglobulin (A2M) ELISA Kit | KTE100313 | Abbkine
Application: This Rat Alpha-2-macroglobulin (A2M) ELISA Kit employs a two-site sandwich ELISA to quantitate A2M in samples. An antibody specific for A2M has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyA2M present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A2M is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A2M bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Alpha-2-macroglobulin microplate
• Rat Alpha-2-macroglobulin standard
• Rat Alpha-2-macroglobulin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Alpha-2-macroglobulin (A2M) ELISA Kit has high sensitivity and excellent specificity for detection of Rat A2M. No significant cross-reactivity or interference between Rat A2M and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Alpha2-macroglobulin is a major serum protein with diverse functions, including inhibition of protease activity and binding of growth factors, cytokines, and disease factors. It is also a panproteinase inhibitor that is found immunohistochemically in neuritic plaques. Alpha-2-macroglobulin is able to inactivate an enormous variety of proteinases (including serine-, cysteine-, aspartic- and metalloproteinases) . Alpha-2-macroglobulin has in its structure a 35 aminoacid "bait" region. Proteinases binding and cleaving the bait region become bound to alpha2M. The proteinase-α2M complex is recognised by macrophage receptors and cleared from the system
Alternative Names: A2M; CPAMD5; DKFZp779B086; FWP007; S863-7;
Search name: A2M; CPAMD5; DKFZp779B086; FWP007; S863-7;
Tag: A2M
