Product Description
Rat Complement fragment 5 (C5) ELISA Kit | KTE100850 | Abbkine
Application: This Rat Complement fragment 5 (C5) ELISA Kit employs a two-site sandwich ELISA to quantitate C5 in samples. An antibody specific for C5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Complement fragment 5 microplate
• Rat Complement fragment 5 standard
• Rat Complement fragment 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Complement fragment 5 (C5) ELISA Kit has high sensitivity and excellent specificity for detection of Rat C5. No significant cross-reactivity or interference between Rat C5 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Complement component 5 is a protein involved in the complement system.C5 is a 190-kD glycoprotein comprised of 2 disulfide-linked polypeptide chains, alpha (C5a) and beta (C5b), with a molecular mass of 155 and 75 kD, respectively (Tack et al., 1979) . Haviland et al. (1991) constructed the complete cDNA sequence of human complement pro-C5, which is predicted to encode a 1, 676-amino acid promolecule that contains an 18-amino acid leader peptide and a 4-amino acid linker separating the beta and alpha chains. Northern blot analysis demonstrated a major 6.0-kb C5 transcript, as well as 3.0-kb and 4.8-kb transcripts. Carney et al. (1991) determined that the C5 gene contains 41 exons that span a genomic region of 79 kb.
Alternative Names: C5; CPAMD4; FLJ17816; FLJ17822; MGC142298; anaphylatoxin C5a analog
Search name: C5; CPAMD4; FLJ17816; FLJ17822; MGC142298; anaphylatoxin C5a analog
Tag: C5