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Product Description
Rat Histone acetyltransferase MYST3 (MYST3) ELISA Kit | KTE100532 | Abbkine
Application: This Rat Histone acetyltransferase MYST3 (MYST3) ELISA Kit employs a two-site sandwich ELISA to quantitate MYST3 in samples. An antibody specific for MYST3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYST3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYST3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYST3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Histone acetyltransferase MYST3 microplate
• Rat Histone acetyltransferase MYST3 standard
• Rat Histone acetyltransferase MYST3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Histone acetyltransferase MYST3 (MYST3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MYST3. No significant cross-reactivity or interference between Rat MYST3 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: KAT6A encodes a member of the MOZ, YBFR2, SAS2, TIP60 family of histone acetyltransferases. The protein is composed of a nuclear localization domain, a double C2H2 zinc finger domain that binds to acetylated histone tails, a histone acetyl-transferase domain, a glutamate/aspartate-rich region, and a serine- and methionine-rich transactivation domain. It is part of a complex that acetylates lysine-9 residues in histone 3, and in addition, it acts as a co-activator for several transcription factors. Allelic variants of KAT6A are associated with autosomal dominant mental retardation-32. Alternative splicing results in multiple transcript variants.
Alternative Names: MYST3; KAT6A; MGC167033; MOZ; RUNXBP2; ZNF220; Monocytic leukemia zinc finger protein; runt-related transcription factor binding protein 2; zinc finger protein 220
Search name: MYST3; KAT6A; MGC167033; MOZ; RUNXBP2; ZNF220; Monocytic leukemia zinc finger protein; runt-related transcription factor binding protein 2; zinc finger protein 220
Tag: MYST3
