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Product Description
Rat Low-density lipoprotein receptor-related protein 2 (LRP2) ELISA Kit | KTE100885 | Abbkine
Application: This Rat Low-density lipoprotein receptor-related protein 2 (LRP2) ELISA Kit employs a two-site sandwich ELISA to quantitate LRP2 in samples. An antibody specific for LRP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Low-density lipoprotein receptor-related protein 2 microplate
• Rat Low-density lipoprotein receptor-related protein 2 standard
• Rat Low-density lipoprotein receptor-related protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Low-density lipoprotein receptor-related protein 2 (LRP2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LRP2. No significant cross-reactivity or interference between Rat LRP2 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: LRP2 is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins.
Alternative Names: LRP2; DBS; gp330; Heymann nephritis antigen homolog; calcium sensor protein; megalin
Search name: LRP2; DBS; gp330; Heymann nephritis antigen homolog; calcium sensor protein; megalin
Tag: LRP2
