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Product Description
Rat Nuclear pore complex protein Nup153 (NUP153) ELISA Kit | KTE100879 | Abbkine
Application: This Rat Nuclear pore complex protein Nup153 (NUP153) ELISA Kit employs a two-site sandwich ELISA to quantitate NUP153 in samples. An antibody specific for NUP153 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNUP153 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NUP153 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NUP153 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Nuclear pore complex protein Nup153 microplate
• Rat Nuclear pore complex protein Nup153 standard
• Rat Nuclear pore complex protein Nup153 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Nuclear pore complex protein Nup153 (NUP153) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NUP153. No significant cross-reactivity or interference between Rat NUP153 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. The protein encoded by NUP153 has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats.
Alternative Names: NUP153; HNUP153; N153; nuclear pore complex protein hnup153
Search name: NUP153; HNUP153; N153; nuclear pore complex protein hnup153
Tag: NUP153
