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Product Description
Rat Odontogenic ameloblast-associated protein (ODAM) ELISA Kit | KTE100878 | Abbkine
Application: This Rat Odontogenic ameloblast-associated protein (ODAM) ELISA Kit employs a two-site sandwich ELISA to quantitate ODAM in samples. An antibody specific for ODAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyODAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ODAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ODAM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Odontogenic ameloblast-associated protein microplate
• Rat Odontogenic ameloblast-associated protein standard
• Rat Odontogenic ameloblast-associated protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Odontogenic ameloblast-associated protein (ODAM) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ODAM. No significant cross-reactivity or interference between Rat ODAM and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: ODAM is expressed in rodent enamel organ and is likely involved in dental development.Notably, these reagents immunostained normal and malignant ameloblasts and other types of human neoplastic cells, including those of gastric, lung, and breast origin where the presence in the latter was confirmed by in situ hybridization using gene-specific molecular probes. Moreover, significant titers of anti-ODAM IgG antibodies were detected in the sera of patients with these malignancies.ODAM is a developmental antigen that has an essential role in tooth maturation and in the pathogenesis of certain odontogenic and other epithelial neoplasms; ODAM may serve as a novel prognostic biomarker, as well as a potential diagnostic and therapeutic target for patients with breast and other epithelial forms of cancer
Alternative Names: ODAM; APIN; FLJ20513; odontogenic ameloblast-associated protein
Search name: ODAM; APIN; FLJ20513; odontogenic ameloblast-associated protein
Tag: ODAM
