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Product Description
Rat Paired box protein Pax-6 (PAX6) ELISA Kit | KTE100499 | Abbkine
Application: This Rat Paired box protein Pax-6 (PAX6) ELISA Kit employs a two-site sandwich ELISA to quantitate PAX6 in samples. An antibody specific for PAX6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAX6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAX6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAX6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Paired box protein Pax-6 microplate
• Rat Paired box protein Pax-6 standard
• Rat Paired box protein Pax-6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Paired box protein Pax-6 (PAX6) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PAX6. No significant cross-reactivity or interference between Rat PAX6 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: PAX6 is the most researched of the Pax genes and appears throughout the literature as a "master control" gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. This transcription factor is most famous for its use in the interspecifically induced expression of ectopic eyes and is of medical importance because heterozygous mutants produce a wide spectrum of ocular defects such as Aniridia in humans. PAX6 protein function is highly conserved across bilaterian species, for instance mouse PAX6 can trigger eye development in Drosophila melanogaster.
Alternative Names: PAX6; AN; AN2; D11S812E; MGC17209; MGDA; WAGR; OTTHUMP00000038834; OTTHUMP00000038836; OTTHUMP00000038837; OTTHUMP00000038838; OTTHUMP00000038839; OTTHUMP00000038840; paired box gene 6; paired box homeotic gene-
Search name: PAX6; AN; AN2; D11S812E; MGC17209; MGDA; WAGR; OTTHUMP00000038834; OTTHUMP00000038836; OTTHUMP00000038837; OTTHUMP00000038838; OTTHUMP00000038839; OTTHUMP00000038840; paired box gene 6; paired box homeotic gene-
Tag: PAX6
