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Product Description
Rat Pancreatic prohormone (PPY) ELISA Kit | KTE101004 | Abbkine
Application: This Rat Pancreatic prohormone (PPY) ELISA Kit employs a two-site sandwich ELISA to quantitate PPY in samples. An antibody specific for PPY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPY bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Pancreatic prohormone microplate
• Rat Pancreatic prohormone standard
• Rat Pancreatic prohormone detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Pancreatic prohormone (PPY) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PPY. No significant cross-reactivity or interference between Rat PPY and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Pancreatic polypeptide (PP) is a 36-amino-acid secretory peptide that is predominantly produced by the pancreas. The exact physiologic role of PP in healthy individuals has not been fully defined. This peptide affects the secretion of pancreatic enzymes, water, and electrolytes. Its effect is biphasic in that PP initially enhances secretion and then inhibits secretion. PP increases gastric emptying and gut motility. It also relaxes the pyloric and ileocecocolic sphincters, the colon, and gallbladder. PP levels increase after ngestion of food and remain elevated from 4-8 hours. Prolonged fasting, diabetes, and exercise can also increase PP levels. Serum PP levels can be elevated in as many as 50% of patients with carcinoid syndrome. Increased levels can also be found in patients with duodenal ulcers and in patients with type I diabetes.
Alternative Names: PPY; PNP; pancreatic polypeptide Y
Search name: PPY; PNP; pancreatic polypeptide Y
Tag: PPY
