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Product Description
Rat Parafibromin (CDC73) ELISA Kit | KTE100820 | Abbkine
Application: This Rat Parafibromin (CDC73) ELISA Kit employs a two-site sandwich ELISA to quantitate CDC73 in samples. An antibody specific for CDC73 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCDC73 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDC73 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDC73 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Parafibromin microplate
• Rat Parafibromin standard
• Rat Parafibromin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Parafibromin (CDC73) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CDC73. No significant cross-reactivity or interference between Rat CDC73 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: CDC73 encodes a tumor suppressor that is involved in transcriptional and post-transcriptional control pathways. The protein is a component of the the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. This protein appears to facilitate the association of 3' mRNA processing factors with actively-transcribed chromatin. Mutations in CDC73 have been linked to hyperparathyroidism-jaw tumor syndrome, familial isolated hyperparathyroidism, and parathyroid carcinoma.
Alternative Names: CDC73; C1orf28; FLJ23316; HPTJT; HRPT2; hyperparathyroidism 2 protein; parafibromin
Search name: CDC73; C1orf28; FLJ23316; HPTJT; HRPT2; hyperparathyroidism 2 protein; parafibromin
Tag: CDC73
