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Product Description
Rat Synaptotagmin-10 (SYT10) ELISA Kit | KTE100153 | Abbkine
Application: This Rat Synaptotagmin-10 (SYT10) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT10 in samples. An antibody specific for SYT10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Synaptotagmin-10 microplate
• Rat Synaptotagmin-10 standard
• Rat Synaptotagmin-10 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Synaptotagmin-10 (SYT10) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SYT10. No significant cross-reactivity or interference between Rat SYT10 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The protein encoded by SYT10 involved in Ca (2+) -dependent exocytosis of secretory vesicles [RGD, Feb 2006].Ca2+ sensor specifically required for the Ca2+-dependent exocytosis of secretory vesicles containing IGF1 in neurons of the olfactory bulb. Exocytosis of IGF1 is required for sensory perception of smell. Not involved in Ca2+-dependent synaptic vesicle exocytosis (By similarity) . Acts through Ca2+ and phospholipid binding to the C2 domain: Ca2+ induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis (By similarity) .
Alternative Names: SYT10; MGC119436; MGC119437; synaptotagmin 10
Search name: SYT10; MGC119436; MGC119437; synaptotagmin 10
Tag: SYT10
