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Product Description
Rat Thymus activation regulated chemokine (TARC) ELISA Kit | KTE101003 | Abbkine
Application: This Rat Thymus activation regulated chemokine (TARC) ELISA Kit employs a two-site sandwich ELISA to quantitate TARC/CCL17 in samples. An antibody specific for TARC/CCL17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTARC/CCL17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARC/CCL17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARC/CCL17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Thymus activation regulated chemokine microplate
• Rat Thymus activation regulated chemokine standard
• Rat Thymus activation regulated chemokine detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Thymus activation regulated chemokine (TARC) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TARC/CCL17. No significant cross-reactivity or interference between Rat TARC/CCL17 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC) . CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.
Alternative Names: TARC/CCL17
Search name: TARC/CCL17
Tag: TARC/CCL17
