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Product Description
Rat Transmembrane protein 171 (TMEM171) ELISA Kit | KTE100098 | Abbkine
Application: This Rat Transmembrane protein 171 (TMEM171) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM171 in samples. An antibody specific for TMEM171 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM171 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM171 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM171 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Transmembrane protein 171 microplate
• Rat Transmembrane protein 171 standard
• Rat Transmembrane protein 171 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Transmembrane protein 171 (TMEM171) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TMEM171. No significant cross-reactivity or interference between Rat TMEM171 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: TMEM171 (transmembrane protein 171) is a 324 amino acid protein encoded by a gene mapping to human chromosome 5. With 181 million base pairs encoding around 1, 000 genes, chromosome 5 is about 6% of human genomic DNA. It is associated with Cockayne syndrome through the ERCC8 gene and familial adenomatous polyposis through the adenomatous polyposis coli (APC) tumor suppressor gene. Treacher Collins syndrome is also chromosome 5 associated and is caused by insertions or deletions within the TCOF1 gene. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome. Deletion of 5q or chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome.
Alternative Names: TMEM171; PRP2; proline-rich protein PRP2
Search name: TMEM171; PRP2; proline-rich protein PRP2
Tag: TMEM171
