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Product Description
Human Double-stranded RNA-specific adenosine deaminase (ADAR) ELISA Kit | KTE60927 | Abbkine
Application: This Human Double-stranded RNA-specific adenosine deaminase (ADAR) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAR in samples. An antibody specific for ADAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Double-stranded RNA-specific adenosine deaminase microplate
• Human Double-stranded RNA-specific adenosine deaminase standard
• Human Double-stranded RNA-specific adenosine deaminase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Double-stranded RNA-specific adenosine deaminase (ADAR) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAR. No significant cross-reactivity or interference between Human ADAR and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Double-stranded RNA-specific adenosine deaminase is an enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.ADAR requires the small molecule inositol hexakisphosphate (IP6) for proper function. Ubiquitously expressed, highest levels were found in brain and lung.SH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.
Alternative Names: ADAR; RP11-61L14.5; ADAR1; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88dsRBP; p136; 136 kDa double-stranded RNA binding protein; adenosine deaminase acting on RNA 1-A; interferon-induced protein 4; interferon-i
Search name: ADAR; RP11-61L14.5; ADAR1; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88dsRBP; p136; 136 kDa double-stranded RNA binding protein; adenosine deaminase acting on RNA 1-A; interferon-induced protein 4; interferon-i
Tag: ADAR
