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Product Description
Human Major prion protein (PRNP) ELISA Kit | KTE61138 | Abbkine
Application: This Human Major prion protein (PRNP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRNP in samples. An antibody specific for PRNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRNP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRNP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Major prion protein microplate
• Human Major prion protein standard
• Human Major prion protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Major prion protein (PRNP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRNP. No significant cross-reactivity or interference between Human PRNP and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: PRNP is a gene that codes for a protein called the prion protein (PrP), which is expressed in the brain and several other tissues.The human PRNP gene is located on the short (p) arm of chromosome 20 between the end (terminus) of the arm and position 12, from base pair 4, 615, 068 to base pair 4, 630, 233. PRNP has also recently been designated CD230 (cluster of differentiation 230) . Although the precise function of PrP is not yet known, it is possibly involved in the transport of ionic copper to cells from the surrounding environment. Researchers have also proposed roles for PrP in cell signaling or in the formation of gaps between nerve cells (synapses) where cell-to-cell communication occurs.
Alternative Names: PRNP; ASCR; CD230; CJD; GSS; MGC26679; PRIP; PrP; PrP27-30; PrP33-35C; PrPc; prion; CD230 antigen; major prion protein; p27-30; prion protein PrP; prion-related protein
Search name: PRNP; ASCR; CD230; CJD; GSS; MGC26679; PRIP; PrP; PrP27-30; PrP33-35C; PrPc; prion; CD230 antigen; major prion protein; p27-30; prion protein PrP; prion-related protein
Tag: PRNP
