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Product Description
Human Proprotein convertase subtilisin/kexin type 5 (PCSK5) ELISA Kit | KTE61286 | Abbkine
Application: This Human Proprotein convertase subtilisin/kexin type 5 (PCSK5) ELISA Kit employs a two-site sandwich ELISA to quantitate PCSK5 in samples. An antibody specific for PCSK5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCSK5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Proprotein convertase subtilisin/kexin type 5 microplate
• Human Proprotein convertase subtilisin/kexin type 5 standard
• Human Proprotein convertase subtilisin/kexin type 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Proprotein convertase subtilisin/kexin type 5 (PCSK5) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCSK5. No significant cross-reactivity or interference between Human PCSK5 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Proprotein convertase subtilisin/kexin type 5 is an enzyme belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein mediates posttranslational endoproteolytic processing for several integrin alpha subunits. It is thought to process prorenin, pro-membrane type-1 matrix metalloproteinase and HIV-1 glycoprotein gp160. Two alternatively spliced transcripts are described for this gene but only one has its full length nature known. Expressed in T-lymphocytes. Likely to represent a widespread endoprotease activity within the constitutive and regulated secretory pathway. Capable of cleavage at the RX (K/R) R consensus motif.
Alternative Names: PCSK5; RP11-422N19.4; PC5; PC6; PC6A; SPC6; prohormone convertase 5; proprotein convertase PC5; protease PC6; subtilisin/kexin-like protease PC5
Search name: PCSK5; RP11-422N19.4; PC5; PC6; PC6A; SPC6; prohormone convertase 5; proprotein convertase PC5; protease PC6; subtilisin/kexin-like protease PC5
Tag: PCSK5
