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Product Description
Human Protein S100-A7A (S100A7A) ELISA Kit | KTE60761 | Abbkine
Application: This Human Protein S100-A7A (S100A7A) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A7A in samples. An antibody specific for S100A7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A7A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A7A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Protein S100-A7A microplate
• Human Protein S100-A7A standard
• Human Protein S100-A7A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Protein S100-A7A (S100A7A) ELISA Kit has high sensitivity and excellent specificity for detection of Human S100A7A. No significant cross-reactivity or interference between Human S100A7A and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The human calcium-binding protein (hS100A15) was first identified in inflamed hyperplastic psoriatic skin, where the S100A15 gene is transcribed into two mRNA splice variants, hS100A15-S and hS100A15-L. In cultured keratinocytes, IL-1beta and Th1 cytokines significantly induced hS100A15-L compared with hS100A15-S. In contrast, Th2-derived cytokines had no influence on the expression of either hS100A15 splice variant. Differentiation of human keratinocytes induced by 1.2 mm calcium resulted in the upregulation of both hS100A15 mRNA isoforms. Overexpression of hS100A15 in chronic inflammatory skin diseases and regulation by inflammatory cytokines and calcium suggest that hS100A15 is involved in Th1-associated epithelial responses and epidermal maturation in normal and diseased human skin.
Alternative Names: S100A7A; NICE-2; S100A15; S100A7L1; S100A7f; OTTHUMP00000015325; S100 calcium binding protein A15; S100 calcium binding protein A7-like 1
Search name: S100A7A; NICE-2; S100A15; S100A7L1; S100A7f; OTTHUMP00000015325; S100 calcium binding protein A15; S100 calcium binding protein A7-like 1
Tag: S100A7A
