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Product Description
Human Replication initiator 1 (REPIN1) ELISA Kit | KTE60806 | Abbkine
Application: This Human Replication initiator 1 (REPIN1) ELISA Kit employs a two-site sandwich ELISA to quantitate REPIN1 in samples. An antibody specific for REPIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREPIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REPIN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REPIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Replication initiator 1 microplate
• Human Replication initiator 1 standard
• Human Replication initiator 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Replication initiator 1 (REPIN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human REPIN1. No significant cross-reactivity or interference between Human REPIN1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Replication initiator 1 is a protein encoded by the REPIN1 gene. Homodimers of RIP60 (replication initiation-region protein 60 kDA) purified from nuclear extract bind two ATT-rich sites in oribeta and foster the formation of a twisted 720 bp DNA loop in vitro. RIP60 contains 15 C (2) H (2) zinc finger (ZF) DNA binding motifs organized in three clusters, termed hand Z1 (ZFs 1-5), hand Z2 (ZFs 6-8) and hand Z3 (ZFs 9-15) . A proline-rich region is located between hands Z2 and Z3. Gel mobility shift and DNase I footprinting experiments show hands Z1 and Z2 independently bind the oribeta RIP60 sites specifically, but with different affinities. Hand Z3 binds DNA, but displays no specificity for RIP60 sites.
Alternative Names: REPIN1; AP4; RIP60; ZNF464; Zfp464; H_DJ0584D14.12; replication initiation region protein (60kD) ; zinc finger protein 464 (RIP60) ; zinc finger protein AP4; zinc finger proten AP4
Search name: REPIN1; AP4; RIP60; ZNF464; Zfp464; H_DJ0584D14.12; replication initiation region protein (60kD) ; zinc finger protein 464 (RIP60) ; zinc finger protein AP4; zinc finger proten AP4
Tag: REPIN1
