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Product Description
Human S-phase kinase-associated protein 1 (SKP1) ELISA Kit | KTE60647 | Abbkine
Application: This Human S-phase kinase-associated protein 1 (SKP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SKP1 in samples. An antibody specific for SKP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySKP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SKP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SKP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human S-phase kinase-associated protein 1 microplate
• Human S-phase kinase-associated protein 1 standard
• Human S-phase kinase-associated protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human S-phase kinase-associated protein 1 (SKP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SKP1. No significant cross-reactivity or interference between Human SKP1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: S-phase kinase-associated protein 1 is a member of the SCF ubiquitin ligase protein complex. It binds to proteins containing an F-box motif, such as cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin dependent proteolysis. The encoded protein also collaborates with a network of proteins to control beta-catenin levels and affects the activity level of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
Alternative Names: SKP1; EMC19; MGC34403; OCP-II; OCP2; SKP1A; TCEB1L; p19A; RNA polymerase elongation factor-like protein OCP2; cyclin A/CDK2-associated p19; organ of Corti protein 2; transcription elongation factor B (SII
Search name: SKP1; EMC19; MGC34403; OCP-II; OCP2; SKP1A; TCEB1L; p19A; RNA polymerase elongation factor-like protein OCP2; cyclin A/CDK2-associated p19; organ of Corti protein 2; transcription elongation factor B (SII
Tag: SKP1
