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Product Description
Human Septin-12 (SEPT12) ELISA Kit | KTE60828 | Abbkine
Application: This Human Septin-12 (SEPT12) ELISA Kit employs a two-site sandwich ELISA to quantitate SEPT12 in samples. An antibody specific for SEPT12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEPT12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEPT12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEPT12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Septin-12 microplate
• Human Septin-12 standard
• Human Septin-12 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Septin-12 (SEPT12) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEPT12. No significant cross-reactivity or interference between Human SEPT12 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Septins are a group of highly conserved GTP binding proteins found in eukaryotes. In yeast cells, they build scaffolding to provide structural support during cell division and compartmentalize parts of the cell. Septins, such as SEPT12, are conserved GTP-binding proteins that function as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeleton remodeling, as well as infection, neurodegeneration, and neoplasia. By searching databases for members of the septin family, Hall et al. (2005) identified SEPT12. DNA microarray analysis showed low expression of SEPT12 in all normal, diseased, and cancer tissues examined.By genomic sequence analysis, Hall et al. (2005) mapped the SEPT12 gene to chromosome 16p13.3.
Alternative Names: SEPT12; FLJ25410;
Search name: SEPT12; FLJ25410;
Tag: SEPT12
