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Product Description
Human Slit homolog 3 protein (SLIT3) ELISA Kit | KTE60580 | Abbkine
Application: This Human Slit homolog 3 protein (SLIT3) ELISA Kit employs a two-site sandwich ELISA to quantitate SLIT3 in samples. An antibody specific for SLIT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLIT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLIT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Slit homolog 3 protein microplate
• Human Slit homolog 3 protein standard
• Human Slit homolog 3 protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Slit homolog 3 protein (SLIT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLIT3. No significant cross-reactivity or interference between Human SLIT3 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The SLIT3 cDNA encodes a 1, 523-amino acid polypeptide with 41.1% similarity to the Drosophila 'slit' protein. Northern blot analysis revealed that the human SLIT3 gene was expressed as a major 5.5- and a minor 9.5-kb transcript primarily in the thyroid. SLIT1 and SLIT2 mRNAs were primarily expressed in the brain and spinal cord, respectively. In situ hybridization studies indicated that the rat Slit1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. These data suggested that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.
Alternative Names: SLIT3; FLJ10764; MEGF5; SLIL2; SLIT1; Slit-3; slit2; slit homolog 3
Search name: SLIT3; FLJ10764; MEGF5; SLIL2; SLIT1; Slit-3; slit2; slit homolog 3
Tag: SLIT3
