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Product Description
Human Sphingosine 1-phosphate receptor type 3 (S1P3) ELISA Kit | KTE60755 | Abbkine
Application: This Human Sphingosine 1-phosphate receptor type 3 (S1P3) ELISA Kit employs a two-site sandwich ELISA to quantitate S1PR3 in samples. An antibody specific for S1PR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS1PR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S1PR3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S1PR3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Sphingosine 1-phosphate receptor type 3 microplate
• Human Sphingosine 1-phosphate receptor type 3 standard
• Human Sphingosine 1-phosphate receptor type 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Sphingosine 1-phosphate receptor type 3 (S1P3) ELISA Kit has high sensitivity and excellent specificity for detection of Human S1PR3. No significant cross-reactivity or interference between Human S1PR3 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Sphingosine-1-phosphate receptor 3 also known as S1PR3 is a G protein-coupled receptor which binds the lipid signaling molecule sphingosine 1-phosphate (S1P) . Hence this receptor is also known as S1P3. This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function.According to these guidelines, a receptor is to be named with the abbreviation of the natural agonist with the highest potency, followed by a subscripted arabic number. Thus they suggested that the designation EDG3 should be changed to S1P3.
Alternative Names: S1PR3; EDG-3; EDG3; FLJ37523; FLJ93220; LPB3; MGC71696; S1P3; G protein-coupled receptor; endothelial differentiation gene-3; S1P receptor EDG3; endothelial differentiation; sphingolipid G-protein-coupled re
Search name: S1PR3; EDG-3; EDG3; FLJ37523; FLJ93220; LPB3; MGC71696; S1P3; G protein-coupled receptor; endothelial differentiation gene-3; S1P receptor EDG3; endothelial differentiation; sphingolipid G-protein-coupled re
Tag: S1PR3
