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Product Description
Human Sulfotransferase 1A1 (SULT1A1) ELISA Kit | KTE60402 | Abbkine
Application: This Human Sulfotransferase 1A1 (SULT1A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SULT1A1 in samples. An antibody specific for SULT1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySULT1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SULT1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SULT1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Sulfotransferase 1A1 microplate
• Human Sulfotransferase 1A1 standard
• Human Sulfotransferase 1A1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Sulfotransferase 1A1 (SULT1A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SULT1A1. No significant cross-reactivity or interference between Human SULT1A1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Phenol sulfotransferase, or PST (EC 2.8.2.1), catalyzes the sulfate conjugation of catecholamines and of phenolic drugs. PST is widely distributed in human and animal tissues that include the blood platelets. Human platelet PST exists in at least a thermolabile, or monoamine-metabolizing (SULT1A3, or STM), form and a thermostable, or phenol-metabolizing, form. Evidence that inheritance contributes to differences in levels of the 2 forms of the enzyme in whites was presented by Reveley et al. (1982), Price et al. (1988), and Van Loon and Weinshilboum (1984) . Anderson and Jackson (1984) showed that the mean basal level of platelet thermostable PST activity in American blacks is significantly higher than the mean basal level in whites, whereas the average platelet thermolabile PST activity does not differ significantly in the 2 groups.
Alternative Names: SULT1A1; HAST1/HAST2; MGC131921; MGC5163; P-PST; PST; ST1A3; STP; STP1; TSPST1; aryl sulfotransferase; phenol-preferring phenol sulfotransferase1; phenol-sulfating phenol sulfotransferase; thermostable phenol s
Search name: SULT1A1; HAST1/HAST2; MGC131921; MGC5163; P-PST; PST; ST1A3; STP; STP1; TSPST1; aryl sulfotransferase; phenol-preferring phenol sulfotransferase1; phenol-sulfating phenol sulfotransferase; thermostable phenol s
Tag: SULT1A1
