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Product Description
Human Thymosin beta-4 (TMSB4X) ELISA Kit | KTE60250 | Abbkine
Application: This Human Thymosin beta-4 (TMSB4X) ELISA Kit employs a two-site sandwich ELISA to quantitate TMSB4X in samples. An antibody specific for TMSB4X has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMSB4X present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMSB4X is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMSB4X bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Thymosin beta-4 microplate
• Human Thymosin beta-4 standard
• Human Thymosin beta-4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Thymosin beta-4 (TMSB4X) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMSB4X. No significant cross-reactivity or interference between Human TMSB4X and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: The predominant form of thymosin, thymosin beta4, is a member of a highly conserved family of actin monomer-sequestering proteins. Beta-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmic pool of free G-actin monomers required for rapid filament elongation and allowing for the flux of monomers between the thymosin-bound pool and F-actin. Thymosin beta4 sequesters actin, holding it in a form that is unable to polymerize. Due to its profusion in the cytosol and its ability to bind ATP G-actin but not F-actin, thymosin beta4 is regarded as the principal actin-sequestering protein. Thymosin beta4 binds ATP G- monomeric actin in a 11 complex where G-actin cannot polymerize.
Alternative Names: TMSB4X; FX; PTMB4; TB4X; TMSB4; OTTHUMP00000022925; OTTHUMP00000022927; OTTHUMP00000022928; prothymosin beta-4; thymosin beta-4; thymosin; beta 4; thymosin; beta 4; X chromosome
Search name: TMSB4X; FX; PTMB4; TB4X; TMSB4; OTTHUMP00000022925; OTTHUMP00000022927; OTTHUMP00000022928; prothymosin beta-4; thymosin beta-4; thymosin; beta 4; thymosin; beta 4; X chromosome
Tag: TMSB4X
