Euro
USD
British Pound
NULL
Product Description
Human Transforming growth factor beta-1-induced transcript 1 protein (TGFB1I1) ELISA Kit | KTE60509 | Abbkine
Application: This Human Transforming growth factor beta-1-induced transcript 1 protein (TGFB1I1) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFB1I1 in samples. An antibody specific for TGFB1I1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGFB1I1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFB1I1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFB1I1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Transforming growth factor beta-1-induced transcript 1 protein microplate
• Human Transforming growth factor beta-1-induced transcript 1 protein standard
• Human Transforming growth factor beta-1-induced transcript 1 protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Transforming growth factor beta-1-induced transcript 1 protein (TGFB1I1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TGFB1I1. No significant cross-reactivity or interference between Human TGFB1I1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: TGFB1I1 is a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. TGFB1I1 is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for TGFB1I1.
Alternative Names: TGFB1I1; ARA55; HIC-5; HIC5; TSC-5; androgen receptor coactivator ARA55; androgen receptor-associated protein of 55 kDa; hydrogen peroxide-inducible clone 5 protein
Search name: TGFB1I1; ARA55; HIC-5; HIC5; TSC-5; androgen receptor coactivator ARA55; androgen receptor-associated protein of 55 kDa; hydrogen peroxide-inducible clone 5 protein
Tag: TGFB1I1
