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Product Description
Human Anti-synaptophysin antibody (SYP-Ab) ELISA Kit | AE62397HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: SYP-Ab
Alternative Name: N/A
Application: ELISA
Range: 0.312-20 ng/mL
Sensitivity: 0.117 ng/mL
Intra-Assay: ≤4.7%
Inter-Assay: ≤8.4%
Recovery: 0, 87
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Competitive ELISA
Analysis Method : Qualitative
Test Principale: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SYP-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated SYP-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled SYP-Ab and unlabeled SYP-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of SYP-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview: Synaptophysin is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has lead to the use of synaptophysin immunostaining for quantification of synapses.The exact function of the protein is unknown: it interacts with the essential synaptic vesicle protein synaptobrevin, but when the synaptophysin gene is experimentally inactivated in animals, they still develop and function normally.Synaptophysin can refer both to the vesicle membrane protein.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
