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Product Description
Rat Anti-thyroid-Peroxidase antibody (TPO-Ab) ELISA Kit | AE62728RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: TPO-Ab
Alternative Name: N/A
Application: ELISA
Range: 0.312-20 ng/mL
Sensitivity: 0.128 ng/mL
Intra-Assay: ≤5.7%
Inter-Assay: ≤10.9%
Recovery: 0, 97
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Competitive ELISA
Analysis Method : Qualitative
Test Principale: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPO-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated TPO-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled TPO-Ab and unlabeled TPO-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TPO-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview: Thyroid peroxidase is an enzyme normally found in the thyroid gland, which plays an important role in the production of thyroid hormones. Fabrication of thyroid hormones is conducted by the enzyme thyroid peroxidase, an integral membrane protein present in the apical (colloid-facing) plasma membrane of thyroid epithelial cells.Thyroid peroxidase catalyzes two sequential reactions: Iodination of tyrosines on thyroglobulin Synthesis of thyroxine or triiodothyronine from two iodotyrosines. Through the action of thyroid peroxidase, thyroid hormones accumulate in colloid, on the surface of thyroid epithelial cells. Remember that hormone is still tied up in molecules of thyroglobulin - the task remaining is to liberate it from the scaffold and secrete free hormone into blood.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
