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Product Description
Rat Proliferating cell nuclear antigen antibody (PCNA-Ab) ELISA Kit | AE62745RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: PCNA-Ab
Alternative Name: N/A
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.059 ng/mL
Intra-Assay: ≤3.5%
Inter-Assay: ≤8.7%
Recovery: 0, 95
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Competitive ELISA
Analysis Method : Qualitative
Test Principale: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCNA-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated PCNA-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled PCNA-Ab and unlabeled PCNA-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PCNA-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview: Proliferating Cell Nuclear Antigen a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. It achieves this processivity by encircling the DNA, thus creating a topological link to the genome. It is an example of a DNA clamp. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
