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Product Description
Human 5-hydroxytryptamine/serotonin IgM antibody (5HT/ST-Ab-IgM) ELISA Kit | AE62835HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: 5HT/ST-Ab-IgM
Alternative Name: N/A
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤4.2%
Inter-Assay: ≤7.4%
Recovery: 0, 96
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Qualitative
Test Principale: This assay employs a two-site sandwich ELISA to quantitate 5HT/ST-Ab-IgM in samples. An antibody specific for 5HT/ST-Ab-IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any5HT/ST-Ab-IgM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5HT/ST-Ab-IgM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5HT/ST-Ab-IgM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Serotonin also called 5-hydroxytryptamine, is primarily found in the gastrointestinal (GI) tract and central nervous system (CNS) of animals. Approximately 81 percent of the human body's total serotonin is located in the enterochromaffin cells in the gut检˥桰˥
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
