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Product Description
Human Anti-titin Antibody (TTN) ELISA Kit | AE63044HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: TTN
Alternative Name: N/A
Application: ELISA
Range: 0.312-20 ng/mL
Sensitivity: 0.113 ng/mL
Intra-Assay: ≤5.8%
Inter-Assay: ≤10.8%
Recovery: 1, 04
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Qualitative
Test Principale: This assay employs a two-site sandwich ELISA to quantitate TTN in samples. An antibody specific for TTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TTN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TTN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Titin is a large abundant protein of striated muscle. A N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere respectively so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Considerable variability exists in the I-band, the M-line and the Z-disc regions of titin. Variability in the I-band region contributes to the differences in elasticity of different titin isoforms and, therefore, to the differences in elasticity of different muscle types. Of the many titin variants identified, five for which complete transcript information is available are described.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
