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Product Description
Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit | AE58052HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PLB1
Alternative Name: PLB; PLB/LIP; lysophospholipase|phospholipase A2
Application: ELISA
Range: 0.31-20 ng/mL
Sensitivity: 0.156 ng/mL
Intra-Assay: ≤3.8%
Inter-Assay: ≤7.8%
Recovery: 0, 89
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PLB1 in samples. An antibody specific for PLB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: By Western blot analysis using an antibody against guinea pig phospholipase B (PLB), Maury et al. (2002) detected a 97-kD polypeptide in the soluble and membrane fractions of human epidermis that was enriched in the soluble fraction. Consistent with these results, they found that PLB activity was greatest in the soluble fraction. Trypsin had no effect on the molecular mass or enzymatic activity, suggesting that the protein they detected was already in an active form. Immunolocalization experiments revealed that PLB is expressed in the entire human epidermis with an accumulation in the dermoepidermis junction. In situ hybridization also indicated expression in the entire epidermis.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
