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Product Description
Human Plasmalemma vesicle-associated protein (PLVAP) ELISA Kit | AE60098HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PLVAP
Alternative Name: FELS; PV-1; PV1; gp68; fenestrated-endothelial linked structure protein; PV-1 protein
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤4.9%
Inter-Assay: ≤9.0%
Recovery: 1, 02
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PLVAP in samples. An antibody specific for PLVAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLVAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLVAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLVAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: PLVAP is a type II integral membrane protein with a short N-terminal cytoplasmic tail, a single-span transmembrane domain, and a large C-terminal extracellular domain consisting mostly of alpha helices. It also contains 2 coiled-coil domains predicted to contribute to dimer formation. The extracellular domain has 4 N-glycosylation sites, a proline-rich region, and regular spacing of cysteine residues. The deduced 439-amino acid mouse Plvap protein contains a consensus casein kinase II phosphorylation site that is not found in the human protein. Rat Plvap contains 2 casein kinase II phosphorylation sites. Northern blot analysis revealed a single PLVAP transcript of about 2.4 kb in pancreas, kidney, placenta, muscle, lung, heart, and liver. No signal was detected in brain or testis.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
