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Product Description
Human Protein FADD (FADD) ELISA Kit | AE61125HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: FADD
Alternative Name: GIG3; MGC8528; MORT1; Fas-associated via death domain|Fas-associating death domain-containing protein|Fas-associating protein with death domain|growth-inhibiting gene 3 protein|mediator of receptor-
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.065 ng/mL
Intra-Assay: ≤7.4%
Inter-Assay: ≤10.6%
Recovery: 1, 1
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate FADD in samples. An antibody specific for FADD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFADD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FADD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FADD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: FADD is an adaptor molecule that bridges the Fas-receptor, and other death receptors, to caspase-8 through its death domain to form the death inducing signaling complex during apoptosis. The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
