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Product Description
Human Protein FAM3B (FAM3B) ELISA Kit | AE61105HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: FAM3B
Alternative Name: 2-21; C21orf11; C21orf76; ORF9; PANDER; PRED44; D21M16SJHU19e|cytokine-like protein 2-21|pancreatic derived factor
Application: ELISA
Range: 0.625-40 ng/mL
Sensitivity: 0.255 ng/mL
Intra-Assay: ≤7.1%
Inter-Assay: ≤10.2%
Recovery: 1, 07
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate FAM3B in samples. An antibody specific for FAM3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFAM3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: The authors predicted that FAM3B forms a 4-helix bundle, and 3-dimensional modeling indicated that 4 cysteines could form 2 disulfide bonds linking helices 1 and 4 and helices 2 and 3. The human and mouse FAM3B proteins share 78% amino acid identity. Northern blot analysis detected FAM3B expression at a high level in pancreas and at lower levels in small intestine and prostate. Immunohistochemical analysis of mouse tissues detected prominent Fam3b staining in the endocrine pancreas, specifically in the islets of Langerhans. Staining was also found in round spermatids within the seminiferous tubules, in the Purkinje cell layer of the cerebellum, and in nerve cell bodies of numerous brainstem nuclei. Transfected Chinese hamster ovary cells secreted Fam3b to the culture medium.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
